The previous focus of the museum was the Prussian history of Wesel and the Rhineland from 1609 to 1947 in connection with the historical location of the fort. With its new concept as the LVR-Niederrheinmuseum Wesel, the museum's thematic programme has been extended in terms of time and limitation to the time period above no longer applies. The regional focus is now more strongly placed on the Lower Rhine region and the Dutch-German border region.
Various holdings for documentation of Brandenburg-Prussian sovereignty in the Rhine Region.
Special collections:

Paintings and graphic works relating to the Prussian dynasty.
Sack-Sethe family archive, documents, paintings and furniture.
Edged-weapon collection focusing on the 19th and 20th centuries.
Graphic works with portraits of the Prussian history of events.
Portrait painting of Lower Rhine elites approx. 1600-1900.
Collection of literature about the Lower Rhine Jewry.
Lower Rhine graphics of the 16th and 17th centuries (focusing on cartography and event history).